5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Internal Thoracic (Mammary) Artery Smooth Muscle Cells
 (HITASMC)
Human intrathoratic artery smooth muscle cells (HITASMC) are derived from the intrathoratic artery. They are cryopreserved at second passage. HITASMC can be cultured and propagated 8 population doublings. Proliferation, migration and invasion of SMC are essential pathogenic processes in the development of a broad spectrum of cardiovascular disorders. A Boyden chamber was employed to characterize the diverging migratory and invasion potential of proliferating and nonproliferating human artery SMCs. The differential gene expression of both phenotypes was analyzed by a cDNA array system. Several candidate genes were identified1. Left internal mammary arteries synthesize endothelium-derived hyperpolarizing factor (EDHF), a short-lived K(+) channel activator that persists after inhibition of nitric oxide (NO) and prostaglandin synthesis. The identity of EDHF is identified as 11,12-epoxyeicosatrienoic acid (11,12-EET) produced by an EDHF synthase CYP450-2C, and accounting for about 40% net endothelial cell relaxation2. Expression of non-degradable IkappaB-alpha might have therapeutic potential in both vascular inflammatory reaction and SMC proliferation3.Using HITSMC, it was found that amolodipine can alter Ca2+ mobilization and inhibit voltage-dependent Ca2+ influx and the resulting ERK 1/2 activation4. In human vascular smooth muscle cells of both saphenous vein and internal thoracic artery, UTP was found to act as an anti-proliferative regulator5.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HITASMC Total Kit

adult

Each kit contains an ampoule of cryopreserved HITASMC  (358-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit. 1 Kit  358K-05a
Cryopreserved HITASMC , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 358-05a

Proliferating HITASMC , adult

In Flasks

Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

359-25a

359-50a

359-75a

Proliferating HITASMC , adult  

In Multiwell Plates

Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.

  6 well 

12 well

24 well

48 well

96 well

359-6Wa

359-12Wa

359-24Wa

359-48Wa

359-96Wa
Smooth Muscle Cell Growth Medium Optimized and ready for use . 500 ml 311-500
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 310-500
Smooth Muscle Cell Growth Supplement   30 ml 311-GS
Smooth Muscle Cell Differentiation Medium   250 ml 311D-250
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution) 100 ml 090K
HITASMC RNA , adult Total RNA prepared from HITASMC

10 μg

25 μg

358-R10a

358-R25a