5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Internal Thoracic (Mammary) Artery Endothelial Cells
 (HITAEC)
Human Internal Thoracic (Mammary) Artery Endothelial Cells (HITAEC) are derived from normal human internal thoracic arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings. 


Study showed that the loss of endothelium-dependent relaxation could dangerously predispose the ITA graft to vasospasm in the post-operative period1. Increased incidence of VCAM-1 and ICAM-1 in the aorta and ITA may partly explain the enhanced atherosclerosis associated with diabetes mellitus2. Contractile responses to angiotensin I and II in the radial artery and internal thoracic artery (ITA) segments were examined in vitro3. Acetylcholine induces endothelium-dependent relaxation of the perforating branch of the ITA by stimulation of mucarinic receptors on the endothelial cells4.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HITAEC Total Kit , adult Each kit contains an ampoule of cryopreserved HITAEC  (308-05a), 500 ml of HITAEC Growth Medium (212-500), and a Subculture Reagent Kit (090k). 1 Kit 308K-05a
Cryopreserved HITAEC , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Endothelial Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 308-05a

Proliferating HITAEC , adult 

In Flasks

Cultured and shipped in flasks at third passage in HITAEC Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

309-25a

309-50a

309-75a

Proliferating HITAEC , adult

In Multiwell Plates

Cultured and shipped in plates at third passage in HITAEC Growth Medium.

  6 well 

12 well

24 well

48 well

96 well

309-6Wa

309-12Wa

309-24Wa

309-48Wa

309-96Wa
HITAEC Growth Medium Optimized and ready for use 500 ml 212-500
HITAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 212K-500
HITAEC Basal Medium Add Growth Supplement before use 500 ml 210-500
HITAEC Growth Supplement    30 ml 212-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HITAEC RNA , adult Total RNA prepared from HITAEC 10 μg 308-R10a
HITAEC RNA , adult Total RNA prepared from HITAEC 25 μg 308-R25a