5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Coronary Artery Smooth Muscle Cells
 (HCASMC)
Human Coronary Artery Smooth Muscle Cells (HCASMC) are derived from the tunica intima and tunica media of normal human, fibrous plaque-free coronary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Smooth muscle cells are present in various amounts in early fatty streaks and they are the predominant type of cells in fibrous plaques1,2,3. Atherosclerosis of the coronary arteries can cause angina pectoris and heart attack. Intimal smooth muscle proliferation plays a key event in the development of atherosclerosis. HCASMC is an ideal cellular model for the investigations of coronary artery diseases, such as alteration of smooth muscle cell differentiation in atherosclerosis4,5.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HCASMC Total Kit , adult Each kit contains an ampoule of cryopreserved HAOSMC(350-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090k). 1 Kit 350K-05a
Cryopreserved HCASMC , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

 1 Ampoule 350-05a

Proliferating HCASMC , adult

In Flasks

Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

351-25a

351-50a

351-75a

Proliferating HCASMC , adult

In Multiwell Plates

 Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.

 6 well

12 well

24 well

48 well

96 well

351-6Wa

351-12Wa

351-24Wa

351-48Wa

351-96Wa
Smooth Muscle Cell Growth Medium Optimized and ready for use. 500 ml 311-500
Smooth Muscle Cell Growth Mediium Kitl Basal Medium & Growth Supplement packaged separately. Yields 500 ml 311K-500
Smooth Muscle Cell Basal Medium Add Growth Supplement before use. 500 ml 310-500
Smooth Muscle Cell Growth Supplement    30 ml 311-GS
Smooth Muscle Cell Differentiation Medium   250 ml 311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HCASMC RNA , adult Total RNA prepared from HCASMC.

10 μg

25 μg

350-R10a

350-R25a