GENESPHERE

 

Direct
incorporation

Aminoallyl

3DNAArray50

label 10 ~20ug of
totalRNA

sometimes20~50ug

yes

yes

Inexpensive

no

no ( seperately )

yes

cDNA base
composition

dependent

dependent

independent

cDNA length

dependent

dependent

independent

Accurate expression

genomic
contamination

genomic contamination

yes

LNA blocker

no

no

yes

Fast, easy Protocol

yes

no

yes

No dye bias

incorporation rate:cy3>cy5

yes

yes

 

Array350RP

The Array 350RP microarray labeling kit for cDNA arrays is the most sensitive and versatile kit Genisphere has ever offered.
It can be used to label tiny quantities of any kind of RNA or DNA, including partially-degraded samples, and produces extremely reproducible results.

Array 50 kit

The Array 50 microarray labeling kit is a sensitive, easy-to-use, inexpensive labeling kit designed specifically for use with total RNA samples.

Submicro

The Submicro microarray labeling kit is a highly sensitive, easy-to-use labeling kit designed specifically for use with total RNA samples.

Submicro EX

The Submicro EX microarray labeling kit is a flexible, easy-to-use labeling kit for total or poly (A) + RNA that comes with a choice of high-sensitivity and extra-fast protocols. (The high-sensitivity protocols require use of Genisphere SCL Spin Columns, A100620, sold separately.)

Submicro Oligo

The Submicro Oligo microarray labeling kit is a flexible, easy-to-use kit for labeling total or poly (A) + RNA for hybridization to oligo arrays. The kit offers a choice of high-sensitivity and extra-easy protocols.

DyeSavo

Prevents the fading of fluorescent dye dues to oxidation or photobleaching

Array 350

The Array 350 microarray labeling kit is a versatile highly sensitive, easy-to-use, labeling kit designed to label Total RNA & purified mRNA samples to be used on either cDNA or Oligo arrays.
The Array 350 kit is essentially 3 kits in one, it should be ordered in place of the Submicro, Submicro EX, and Submicro Oligo kits.The Submicro kits will still be available, but we feel the Array 350 kit provides a better labeling solution to our customers.
We have priced it at a reduced cost in comparison to our Submicro kits.The Array 350 kits will also be running a special distribution promotion for the first 3 months (more details to follow).
 
     Array 350RP
Description:

The Array 350RP microarray labeling kit for cDNA arrays is the most sensitive and versatile kit Genisphere has ever offered. It can be used to label tiny quantities of any kind of RNA or DNA, including partially-degraded samples, and produces extremely reproducible results.
Features and benefits:
Unprecedented sensitivity
  Detects low expression messages in about 0.5 to 2 µg of total RNA, or 25 to 100 ng of mRNA or aRNA (amplified RNA)
Exceptional flexibility
  Can be used to label all kinds of samples:
* Total RNA, mRNA and aRNA
* Prokaryotic and eukaryotic RNA
* DNA samples for CGH arrays
* Partially - degraded samples
Excellent reproducibility
  Expression changes of 50% are significant with greater than 99% confidence
 
     Array 50 kit
Description:

The Array 50 Expression Array Detection kit is a highly sensitive, easy-to-use labeling kit designed specifically for use with total RNA samples. Each kit contains sufficient fluorescently-labeled 3DNA dendrimer, nucleotides and buffers to label and hybridize 10 or 20 samples.
Features and benefits:
Excellent sensitivity
  Detects low expression messages in about 10 to 20 µg of total RNA
Fast, easy protocol
  Reduces total hands on time for labeling to about 30 minutes
Attractive pricing
  Combines the convenience of a kit with the cost of a home brew labeling system
Accurate expression measurement
  Unaffected by genomic contamination, which can cause conventional labeling methods to produce false expression data
   
 

Comparison of Features

Array 50

Amino-Allyl

Direct Incorporation

Will not label genomic contamination    
-
Works with about 15µg of total RNA  
-
Costs about $15 per assay  
-
Not susceptible to dye incorporation bias  
-
Contains LNA blocker for superior blocking    
-
Uses simple, time-saving protocol  
-
* Only for home-brew kits made by purchasing reagents separately
 
     Submicro
Description:

The Submicro Expression Array Detection kit is a highly sensitive, easy-to-use labeling kit designed specifically for use with total RNA samples. Each kit contains sufficient fluorescently-labeled 3DNA dendrimer, RT enzyme, nucleotides and buffers to label and hybridize 10 samples.
Features and benefits:
High Sensitivity
  Detects low expression messages in about 1 to 5 µg of total RNA
Fast, easy protocol
  Reduces total "hands on" time to about 30 minutes
4 - channel system
  Allows hybridization of four samples to each array, cutting by two-thirds the number of arrays and hybridizations needed
Accurate expression measurement
  Unaffected by genomic contamination, which can cause conventional labeling methods to produce false expression data
 
     Submicro EX
Description:

The Submicro EX Expression Array Detection Kit is a flexible, easy-to-use labeling kit for total or poly (A) + RNA that comes with a choice of high-sensitivity and extra-fast protocols. (The high-sensitivity protocols require use of Genisphere SCL Spin Columns, A100620, sold separately.) Each kit contains sufficient fluorescently-labeled 3DNA dendrimer, RT enzyme, nucleotides and buffers to label and hybridize 10 samples
Features and benefits:
High Sensitivity
  Detects low expression messages in about 1 to 5 µg of total RNA or 50 to 250 ng of poly (A)+ RNA
Fast, easy protocol
  Offers extra-easy protocol options for use with moderate RNA quantities (20 µg of total or 1 µg of poly (A)+ RNA)
4 - channel system
  Allows hybridization of four samples to each array, cutting by two-thirds the number of arrays and hybridizations needed
Accurate expression measurement
  Unaffected by genomic contamination, which can cause conventional labeling methods to produce false expression data
 
     Submicro Oligo
Description:

The Submicro Oligo Expression Array Detection Kit is a flexible, easy-to-use kit for labeling total or poly (A) + RNA for hybridization to oligo arrays. The kit offers a choice of high-sensitivity and extra-easy protocols.
Features and benefits:
High Sensitivity
  Detects low expression messages in about 1 to 5 µg of total RNA or 50 to 250 ng of poly (A) + RNA
Fast, easy protocol
  Offers extra-easy protocol options for use with moderate RNA quantities (20 µg of total or 1 µg of poly (A)+ RNA)
4 - channel system
  Allows hybridization of four samples to each array, cutting by two-thirds the number of arrays and hybridizations needed
Accurate expression measurement
  Unaffected by genomic contamination, which can cause conventional labeling methods to produce false expression data
 
     Array 350
Description:

Proprietary indirect labeling system for both cDNA and oligo arrays The kit is easy to use, because it employs the same simple protocol for every array. The kit's exquisite sensitivity, proprietary LNA blocker and freedom from dye incorporation bias combine to give you differential data superior to that produced by any conventional labeling method.
Features and benefits:
High Sensitivity
  Detects low expression messages in about 1 to 5 g of eukaryotic total RNA or 50 to 250 ng of poly(A)+ RNA
Fast, easy protocol
  Reduces total hands on time for labeling microgram quantities of total RNA to about 30 minutes, or for labeling larger RNA samples to about 15 minutes
Unprecedented reproducibility
  Capable of detecting expression changes as small as 40% to a 99% confidence level
Accurate expression measurement
  Produces data unaffected by genomic contamination and polyA/polyT
binding, which can cause conventional labeling methods to produce
inaccurate expression measurements