5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Coronary Artery
 (HCAEC)
Human Coronary Artery Endothelial Cells (HCAEC) are isolated from normal human coronary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. The endothelium are crucially involved in the regulation of coronary blood flow and cardiac functions, such as thrombosis1,2, atherosclerosis3, and hypertension4. Strategically located between the circulating blood and the vascular smooth muscle, endothelial cells release numerous vasoactive substances regulating the function of vascular smooth muscle and trafficking blood cells5. HCAEC is an ideal candidate for the studies of endothelial cell metabolism and functional vasodilators, such as prostacyclin, bradykinin, nitric oxide and independent of the former endothelium-derived hyperpolarizing factor5. Co-culturing HCAEC with human coronary artery smooth muscle cells (HCASMC) could generate an in vitro model for the cell-cell interaction system3.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HCAEC Total Kit, adult

 

 

 Each kit contains an ampoule of cryopreserved HCAEC  (300-05a), 500 ml of HCAEC Growth Medium (212-500), and a Subculture Reagent Kit (090K). 1 Kit 300K-05a
Cryopreserved HCAEC, adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 300-05a

Proliferating HCAEC, adult

In Flasks

 Cultured in flasks at third passage in HCAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

301-25a

301-50a

301-75a

Proliferating HCAEC, adult

In Multiwell Plates

Cultured in plates at third passage in HCAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.

6 well 

12 well

24 well

48 well

96 well

301-6Wa

301-12Wa

301-24Wa

301-48Wa

301-96Wa

HCAEC Growth Medium Optimized and ready for use 500 ml 212-500
HCAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 212K-500
HCAEC Basal Medium Add Growth Supplement before use 500 ml 210-500
HCAEC Growth Supplement   30 ml 212-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HCAEC RNA, adult

HCAEC RNA, adult

Total RNA prepared from HCAEC

Total RNA prepared from HCAEC

 10 μg

25 μg 		300-R10a

300-R25a