5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Aortic Smooth Muscle Cells
 (HAOSMC)
Human Aortic Smooth Muscle Cells (HAOSMC ) are derived from tunica intima and tunica media of normal human, fibrous plaque-free aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Arterial smooth muscle cells are capable of synthesizing collagen, elastin, myosin and glycosaminoglycan1,2. Increased production of connective tissue components, hyperplasia and hypertrophy of intimal smooth muscle cells are found to gradually occlude the vessel lumen in atherosclerosis1,2. HAOSMC respond to various factors by proliferating or differentiating3,4. They are a well established cell system for the study of human vascular disorders such as atherosclerosis5,6 and stroke.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HAOSMC Total Kit , adult Each kit contains an ampoule of cryopreserved HAOSMC  (354-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090k). 1 Kit 354K-05a
Cryopreserved HAOSMC , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 354-05a

Proliferating HAOSMC , adult

In Flasks

 Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

355-25a

355-50a

355-75a

Proliferating HAOSMC , adult  

In Multiwell Plates

Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.

  6 well 

12 well

24 well

48 well

96 well

355-6Wa

355-12Wa

355-24Wa

355-48Wa

355-96Wa

Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 310-500
Smooth Muscle Cell Growth Supplement   30 ml 311-GS
Smooth Muscle Cell Differentiation Medium   250 ml 311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HAOSMC RNA , adult Total RNA prepared from HAOSMC

10 μg

25 μg

354-R10a

354-R25a