5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Aortic Endothelial Cells
 (HAOEC)
Human Aortic Endothelial Cells (HAOEC ) are primary endothelial cells isolated from normal human aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. They respond to cytokine stimulation by expressing cell adhesion molecules. Aortic endothelial cells have been used as an in vitro model for the study of morphological and ultra-structural changes 1 in the development of atherosclerosis. HAOEC system has also been used for the study of endothelial cell-dependent lymphocyte activation2 and chemoattractant activity for lymphocytes3 in relation to the degree of atherosclerosis.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HAOEC Total Kit

adul

 

 

 Each kit contains an ampoule of cryopreserved HAOEC  (304-05a), 500 ml of Endothelial Cell Growth Medium (211-500), and a Subculture Reagent Kit (090K). 1 Kit 304K-05a

Cryopreserved HAOEC

adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

 1 Ampoule 304-05a

Proliferating HAOEC , adult 

In Flasks

 Cultured in flasks at third passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

305-25a

305-50a

305-75a

Proliferating HAOEC , adult

In Multiwell Plates

 Cultured in plates at third passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.

  6 well 

12 well

24 well

48 well

96 well

305-6Wa

305-12Wa

305-24Wa

305-48Wa

305-96Wa
Endothelial Cell Growth Medium Optimized and ready for use 500 ml 211-500
Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 211K-500
Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 210-500
Endothelial Cell Growth Supplement   15 ml 211-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HAOEC RNA , adult Total RNA prepared from HAOEC

10 μg

25 μg

304-R10a

304-R25a