5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Subclavian Artery Smooth Muscle Cells
 (HScASMC)
Human subclavian artery smooth muscle cells (HScASMC ) are derived from the subclavian artery. They are cryopreserved at second passage. HScASMC can be cultured and propagated 12 population doublings.

 

Morphology and growth characteristics of HScASMC from neonates were found to be different from those of adults1. A rabbit model using the subclavian artery was developed to study the temporal development and characteristics of an intimal cellular response and functional changes following balloon angioplasty injury2. Prolonged treatment with r-hirudin neither inhibits vascular smooth muscle cell proliferation in rabbits after angioplasty of the left subclavian artery nor reduces the size of neointimal lesion. This suggests r-hirudin treatment is not suitable as an antirestenotic intervention3. Subclavian-pulmonary artery anastomoses were created in Sprague-Dawley rats under different experimental conditions. It was found that the type of injury and the associated hemodynamic conditions could modify the elastic pulmonary response to injury4.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HScASMC Total Kit, adult Each kit contains an ampoule of cryopreserved HScASMC  (3510-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K). 1 Kit 3510K-05a
Cryopreserved HScASMC , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 3510-05a

Proliferating HScASMC , adult

In Flasks

Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

3511-25a

3511-50a

3511-75a

Proliferating HScASMC , adult  

In Multiwell Plates

Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.

  6 well 

12 well

24 well

48 well

96 well

3511-6Wa

3511-12Wa

3511-24Wa

3511-48Wa

3511-96Wa

Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 310-500
Smooth Muscle Cell Growth Supplement   30 ml 311-GS
Smooth Muscle Cell Differentiation Medium   250 ml 311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K