5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Pulmonary Artery Smooth Muscle Cells
 (HPASMC)
Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are derived from tunica intima and tunica media of normal human, fibrous plaque-free pulmonary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. HPASMC are well suited for the study of pulmonary vascular disorders1, associated liver diseases2, and connective tissue diseases3. Phenotypic changes of smooth muscle cells have been found to be related to smooth muscle cell migration and intimal proliferation. Switches in smooth muscle cell phenotype lead to pulmonary hypertension and eventually heart and lung disorders.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HPASMC Total Kit , adult Each kit contains an ampoule of cryopreserved HPASMC  (352-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit. 1 Kit 352K-05a
Cryopreserved HPASMC , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 352-05a

Proliferating HPASMC , adult

In Flasks

 Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

353-25a

353-50a

353-75a

Proliferating HPASMC , adult

In Multiwell Plates

 Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.

  6 well 

12 well

24 well

48 well

96 well

353-6Wa

353-12Wa

353-24Wa

353-48Wa

353-96Wa

Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 310-500
Smooth Muscle Cell Growth Supplement   30 ml 311-GS
Smooth Muscle Cell Differentiation Medium   250 ml 311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HPASMC RNA , adult Total RNA prepared from HPASMC

10 μg

25 μg

352-R10a

352-R25a