5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Cardiac Fibroblasts
 (HCF)
Human Cardiac Fibroblasts (HCF) are derived from normal human heart tissue. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings.

HCF has been used to study the effects of TNF-alpha and angiotensin (Ang) II levels on fibroblast function1. Expression of MyoD can induce myogenesis of cardiac fibroblasts2. Cardiac fibroblasts involved in aortic regurgitation synthesize more Fibronectin3. Estradiol inhibits cardiac fibroblast growth through conversion to methoxyestradiols, which may protect against remodeling involved in heart disease4. TGF-beta stimulates differentiation of fibroblasts to myofibroblasts, which increases collagen production5. Serum depletion of fibroblasts simulates one of the components of ischemia6.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HCF Total Kit

fetal

 Each kit contains an ampoule of cryopreserved HCF (306-05f), 500 ml of HCF Growth Medium (516-500), and a Subculture Reagent Kit (090k). 1 Kit 306K-05f

Cryopreserved HCF

fetal

(>500,000 cells in an ampoule)

Cryopreserved at first passage in HCF Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 306-05f

Proliferating HCF

fetal

In Flasks

 Cultured and shipped in flasks at second passage in HCF Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

307-25f

307-50f

307-75f

Proliferating HCF

fetal

In Multiwell Plates

 Cultured and shipped in plates at second passage in HCF Growth Medium.

  6 well

12 well

24 well

48 well

96 well

 307-6Wf

307-12Wf

307-24Wf

307-48Wf

307-96Wf

HCF Growth Medium Optimized and ready for use. 500 ml 516-500
HCF Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 516K-500
HCF Basal Medium Add Growth Supplement before use. 500 ml 515-500
HCF Growth Supplement    50 ml 516-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K

HCF RNA

fetal

 Total RNA prepared from HCF

10 μg

25 μg

306-R10f

306-R25f