5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Subclavian Artery Endothelial Cells
 (HScAEC)
Human Subclavian Artery Endothelial Cells (HScAEC ) are derived from normal human subclavian arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.

The shape and orientation of HScAEC was found to be strongly affected by blood flow conditions1. Autologous leukocyte adhesion to normal and pathologically altered subclavian arteries ex vivo was explored2. The effect of prolonged treatment with r-hirudin after angioplasty of the subclavian artery was evaluated in rabbits3, whereas rabbit ScAEC was used to study the effects of dietary and genetically induced hypercholesterolaemia on the vasodilator and antiaggregatory capacity of the endothelium 4.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HScAEC Total Kit, adult Each kit contains an ampoule of cryopreserved HScAEC (3010-05a), 500 ml of HScAEC Growth Medium (212-500), and a Subculture Reagent Kit (090K). 1 Kit 3010K-05a
Cryopreserved HScAEC, adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 3010-05a

Proliferating HScAEC, adult

In Flasks

 Cultured and shipped in flasks at third passage in HScAEC Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

3011-25a

3011-50a

3011-75a

Proliferating HScAEC, adult  

In Multiwell Plates

 Cultured and shipped in plates at third passage in HScAEC Growth Medium.

  6 well 

12 well

24 well

48 well

96 well

3011-6Wa

3011-12Wa

3011-24Wa

3011-48Wa

3011-96Wa
HScAEC Growth Medium Optimized and ready for use 500 ml 212-500
HScAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 212K-500
HScAEC Basal Medium Add Growth Supplement before use 500 ml 210-500
HScAEC Growth Supplement   30 ml 212-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HScAEC RNA, adult Total RNA prepared from HScAEC 10 μg 3010-R10a
HScAEC RNA, adult Total RNA prepared from HScAEC 25 μg 3010-R25a