5. Cardiac Cells
[ HAOEC ] [ HCAEC ] [ HCF ] [ HPAEC ] [ HCASMC ] [ HPASMC ]
[ HAOSMC ] [ HITAEC ] [ HITASMC ] [ HScAEC ] [ HScASMC ]  
 
Human Pulmonary Artery Endothelial Cells
 
(HPAEC)
Human Pulmonary Artery Endothelial Cells (HPAEC) are isolated from normal human pulmonary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. HPAEC possess an array of enzymatic activities1,2. They respond to a wide range of vasoactive substances3, commensurate with their control of blood pressure and blood pH in vivo . HPAEC have been used for the study of vascular permeability4 and inflammatory responses5. HPAEC in co-culture with HPASMC have been used as a model for pulmonary angiopathy6.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HPAEC Total Kit, adult

Each kit contains an ampoule of cryopreserved HPAEC (302-05a), 500 ml of Endothelial Cell Growth Medium (211-500), and a Subculture Reagent Kit (090K). 1 Kit 302K-05a

Cryopreserved HPAEC , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 302-05a

Proliferating HPAEC , adult

In Flasks

Cultured in flasks at third passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

303-25a

303-50a

303-75a

Proliferating HPAEC , adult

In Multiwell Plates

Cultured in plates at third passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium

  6 well 

12 well

24 well

48 well

96 well

303-6Wa

303-12Wa

303-24Wa

303-48Wa

303-96Wa

Endothelial Cell Growth Medium Optimized and ready for use 500 ml 211-500
Endothelial Cell Growth Medium Kitl Basal Medium & Growth Supplement packaged separately Yields 500 ml 211K-500
Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 210-500
Endothelial Cell Growth Supplement   15 ml 211-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HPAEC RNA , adult Total RNA prepared from HPAEC 10¥ìg 302-R10a
HPAEC RNA , adult Total RNA prepared from HPAEC 25¥ìg 302-R25a