4. Bone/Joint/Skeletal Cells
[ HC ] [ RD-HC ] [ HFLS ] [ HFLS-OA ] [ HFLS-RA ] [ HOb ] [ HSkMC ]
 
Human Skeletal Muscle Cells
(HSkMC)
Human Skeletal Muscle Cells (HSkMC) are isolated from the limbal skeletal muscle.  They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings.  Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments.  HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia1.   HSkMC also have the potential to act as a cardiac graft, mending damage to the heart2.  Recently, cultured Human Skeletal Muscle cells have been used in micro gravity experiments to study the effects of low gravity environments on Human Skeletal Muscle3.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HSkMC Total Kit, , fetal

 Each kit contains an ampoule of cryopreserved HSkMC (150-05f), 500 ml of Skeletal Muscle Cell Growth Medium (151-500), and a Subculture Reagent Kit. 1 Kit 150K-05f  

Cryopreserved HSkMC , fetal

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Skeletal Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

 1 Ampoule 150-05f

Proliferating HSkMC , fetal

In Flasks

Cultured in flasks at third passage in Skeletal Muscle Cell Growth Medium and shipped in Skeletal Muscle Cell Transfer Medium

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

151-25f

151-50f

151-75f

Proliferating HSkMC , fetal

In Multiwell Plates

 Cultured in plates at third passage in Skeletal Muscle Cell Growth Medium and shipped in Skeletal Muscle Cell Transfer Medium

  6 well

12 well

24 well

48 well

96 well

151-6Wf

151-12Wf

151-24Wf

151-48Wf

151-96Wf
Skeletal Muscle Cell Growth Medium Optimized and ready for use 500 ml 151-500
Skeletal Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 151K-500
Skeletal Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 150-500
Skeletal Muscle Cell Growth Supplement   50 ml x 2 151-GS
Skeletal Muscle Cell Differentiation Medium   250 ml 151D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K

HSkMC RNA , fetal

Differentiated HSkMC Total RNA , fetal

Total RNA prepared from HSkMC Multinucleated Myotubes. 10 μg

10 μg		 150-R10f

150D-R10f
HSkMC RNA , fetal

Differentiated HSkMC Total RNA , fetal		 Total RNA prepared from HSkMC Multinucleated Myotubes. 25 μg

25 μg 		150-R25f

150D-R25f