4. Bone/Joint/Skeletal Cells
[ HC ] [ RD-HC ] [ HFLS ] [ HFLS-OA ] [ HFLS-RA ] [ HOb ] [ HSkMC ]
 
Human Fibroblast-Like Synoviocytes
Human Fibroblast-Like Synoviocytes-Normal Tissue (HFLS-RA)
Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doublings. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation1,2. Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS3, 4. Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations5, 6. We provide two types of HFLS that are useful cellular models for studying the differences between HFLS-RA and HFLS-OA, such as the expression and regulation of proteases7 and integrin subunits8.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HFLS Total Kit normal adult tissue

HFLS-OA Total Kit, adult

HFLS-RA Total Kit, adul

Each kit contains an ampoule of cryopreserved HFLS (400-05a or 404-05a), 500 ml of Synoviocyte Growth Medium (415-500), and a Subculture Reagent Kit (090K). 1 Kit

408K-05a

408OAK-05a

408RAK-05a

Cryopreserved HFLS, normal adult

Cryopreserved HFLS-OA , adult

Cryopreserved HFLS-RA , adult

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Synoviocyte Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 408-05a

408OA-05a

408RA-05a
Proliferating HFLS normal tissue Cultured and shipped in flasks at third passage in Synoviocyte Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

409-25a

409-50a

409-75a

Proliferating HFLS-OA , adult

In Flasks

Cultured and shipped in flasks at third passage in Synoviocyte Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

409OA-25a

409OA-50a

409OA-75a

Proliferating HFLS-RA , adult

In Flasks

Cultured and shipped in flasks at third passage in Synoviocyte Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

409RA-25a

409RA-50a

409RA-75a

Proliferating HFLS , normal tissue

In Multiwell Plates

Cultured and shipped in plates at third passage in Synoviocyte Growth Medium.

  6 well

12 well

24 well

48 well

96 well

409-6Wa

409-12Wa

409-24Wa

409-48Wa

409-96Wa

Proliferating HFLS-OA , adult

In Multiwell Plates

Cultured and shipped in plates at third passage in Synoviocyte Growth Medium.

  6 well

12 well

24 well

48 well

96 well

409OA-6Wa

409OA-12Wa

409OA-24Wa

409OA-48Wa

409OA-96Wa

Proliferating HFLS-RA , adult

In Multiwell Plates

Cultured and shipped in plates at third passage in Synoviocyte Growth Medium.

  6 well

12 well

24 well

48 well

96 well

409RA-6Wa

409RA-12Wa

409RA-24Wa

409RA-48Wa

409RA-96Wa

Synoviocyte Growth Medium Optimized and ready for use 500 ml 415-500
Synoviocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields
500 ml
415K-500
Synoviocyte Basal Medium Add Growth Supplement before use 500 ml 414-500
Synoviocyte Growth Supplement     50 ml 415-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HFLS RNA normal adult tissue

HFLS-OA RNA , adult

HFLS-RA RNA , adult

Total RNA prepared from HFLS

Total RNA prepared from HFLS-OA

Total RNA prepared from HFLS-RA

10 ¥ìg

10
¥ìg


10
¥ìg

408-R10a

408OA-R10a

408RA-R10a
HFLS RNA normal adult tissue

HFLS-OA RNA , adult

HFLS-RA RNA , adult

Total RNA prepared from HFLS

Total RNA prepared from HFLS-OA

Total RNA prepared from HFLS-RA

25 ¥ìg

25 ¥ìg

25 ¥ìg

408-R25a

408OA-R25a

408RA-R25a