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4. Bone/Joint/Skeletal Cells

Human Fibroblast-Like Synoviocytes
Human Fibroblast-Like Synoviocytes-Normal Tissue (HFLS)

Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doublings. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation^1,2. Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS^{3, 4}. Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations^{5, 6}. We provide two types of HFLS that are useful cellular models for studying the differences between HFLS-RA and HFLS-OA, such as the expression and regulation of proteases⁷ and integrin subunits⁸.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HFLS Total Kit normal adult tissue HFLS-OA Total Kit, adult HFLS-RA Total Kit, adul	Each kit contains an ampoule of cryopreserved HFLS (400-05a or 404-05a), 500 ml of Synoviocyte Growth Medium (415-500), and a Subculture Reagent Kit (090K).	1 Kit	408K-05a 408OAK-05a 408RAK-05a
Cryopreserved HFLS, normal adult Cryopreserved HFLS-OA , adult Cryopreserved HFLS-RA , adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Synoviocyte Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	408-05a 408OA-05a 408RA-05a
Proliferating HFLS normal tissue	Cultured and shipped in flasks at third passage in Synoviocyte Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	409-25a 409-50a 409-75a
Proliferating HFLS-OA , adult In Flasks	Cultured and shipped in flasks at third passage in Synoviocyte Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	409OA-25a 409OA-50a 409OA-75a
Proliferating HFLS-RA , adult In Flasks	Cultured and shipped in flasks at third passage in Synoviocyte Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	409RA-25a 409RA-50a 409RA-75a
Proliferating HFLS , normal tissue In Multiwell Plates	Cultured and shipped in plates at third passage in Synoviocyte Growth Medium.	6 well 12 well 24 well 48 well 96 well	409-6Wa 409-12Wa 409-24Wa 409-48Wa 409-96Wa
Proliferating HFLS-OA , adult In Multiwell Plates	Cultured and shipped in plates at third passage in Synoviocyte Growth Medium.	6 well 12 well 24 well 48 well 96 well	409OA-6Wa 409OA-12Wa 409OA-24Wa 409OA-48Wa 409OA-96Wa
Proliferating HFLS-RA , adult In Multiwell Plates	Cultured and shipped in plates at third passage in Synoviocyte Growth Medium.	6 well 12 well 24 well 48 well 96 well	409RA-6Wa 409RA-12Wa 409RA-24Wa 409RA-48Wa 409RA-96Wa
Synoviocyte Growth Medium	Optimized and ready for use	500 ml	415-500
Synoviocyte Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	415K-500
Synoviocyte Basal Medium	Add Growth Supplement before use	500 ml	414-500
Synoviocyte Growth Supplement		50 ml	415-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HFLS RNA normal adult tissue HFLS-OA RNA , adult HFLS-RA RNA , adult	Total RNA prepared from HFLS Total RNA prepared from HFLS-OA Total RNA prepared from HFLS-RA	10 μg 10 μg 10 μg	408-R10a 408OA-R10a 408RA-R10a
HFLS RNA normal adult tissue HFLS-OA RNA , adult HFLS-RA RNA , adult	Total RNA prepared from HFLS Total RNA prepared from HFLS-OA Total RNA prepared from HFLS-RA	25 μg 25 μg 25 μg	408-R25a 408OA-R25a 408RA-R25a