4. Bone/Joint/Skeletal Cells
[ HC ] [ RD-HC ] [ HFLS ] [ HFLS-OA ] [ HFLS-RA ] [ HOb ] [ HSkMC ]
 
Human Chondrocytes
 (HC)
Human Chondrocytes (HC ) are derived from normal human articular cartilage. They are cryopreserved at the second passage and can be cultured and propagated at least 10 population doublings. Chondrocytes produce and maintain the extracellular matrix of cartilage.1 Chondrocytes grow in serial monolayer culture lose their phenotypic markers and became de-differentiated2. This de-differentiation stage can be reversed by culturing them in semi-solid gel3,4. HC has served as useful model for studying chondrocyte differentiation and the effects of cytokines and growth factors on the suppression and maintenance of differentiated cartilage phenotype5.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi
Product Name Product Description Size (vol) Cat. No.
HC Total Kit

adult

feta

 

 Each kit contains an ampoule of cryopreserved HC (402-05), 500 ml of Chondrocyte Growth Medium (411-500), and a Subculture Reagent Kit (090K). 1 Kit

402K-05a

402K-05f

Cryopreserved HC

adult

fetal

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Chondrocyte Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

402-05a

402-05f

Proliferating HC

In Flasks

 

* Specify adult (a) or fetal (f)

Cultured and shipped in flasks at third passage in Chondrocyte Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

403-25a; 403-25f *

403-50a; 403-50f *

403-75a; 403-75f *

Proliferating HC  

In Multiwell Plates

 

* Specify adult (a) or fetal (f)

 Cultured and shipped in plates at third passage in Chondrocyte Growth Medium.

6 well 

12 well

24 well

48 well

96 well

403-6Wa;   403-6Wf *

403-12Wa; 403-12Wf *

403-24Wa; 403-24Wf *

403-48Wa; 403-48Wf *

403-96Wa; 403-96Wf *

Chondrocyte Growth Medium Optimized and ready for use 500 ml 411-500
Chondrocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 411K-500
Chondrocyte Basal Medium Add Growth Supplement before use 500 ml 410-500
Chondrocyte Growth Supplement   50 ml 411-GS
Chondrocyte Differentiation Medium   250 ml 411D-250
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution) 100 ml 090K

HC RNA

adult

fetal

Total RNA prepared from HC 10 μg

402-R10a

402-R10f