7. Rat Cells
[ RAOEC ] [ RAOSMC ] [ RCm ] [ RCF ] [ RDF ] [ REK ] [ RH ] [ RLF ]
 
Rat Aortic Smooth Muscle Cells
 (RAOSMC)

Rat Aortic Smooth Muscle Cells (RAOSMC) are derived from the tunica intima and tunica media of healthy, fibrous plaque-free rat aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Increased arterial smooth muscle cell mass are found in the intimal lesion of atherosclerosis1. Rat aortic smooth muscle cells respond to various factors by cell proliferation2 and hypertrophy3, which are prominent indicators of atherosclerosis in vascular diseases. RAOSMC are well suited for the study of large vessel smooth muscle cell growth and differentiation4,5 and serve as in vitro model in correlation with live rat models.


Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol) Cat. No.
RAOSMC Total Kit Each kit contains an ampoule of cryopreserved RAOSMC (R354-05), 500 ml of Rat SMC Growth Medium (R311-500), and a Subculture Reagent Kit (090K). 1 Kit R354K-05
Cryopreserved RAOSMC

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Rat SMC Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule R354-05

Proliferating RAOSMC

In Flasks

Cultured and shipped in flasks at third passage in Rat SMC Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

R355-25

R355-50

R355-75

Proliferating RAOSMC

In Multiwell Plates

Cultured and shipped in plates at third passage in Rat SMC Growth Medium.

  6 well

12 well

24 well

48 well

96 well

R355-6W

R355-12W

R355-24W

R355-48W

R355-96W

Rat SMC Growth Medium Optimized and ready for use 500 ml R311-500
Rat SMC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml R311K-500
Rat SMC Basal Medium Add Growth Supplement before use 500 ml R310-500
Rat SMC Growth Supplement     50 ml R311-GS
Rat SMC Differentiation Medium   250 ml R311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
RAOSMC RNA Total RNA prepared from RAOSMC

10 μg

25 μg

R354-R10

R354-R25