7. Rat Cells
[ RAOEC ] [ RAOSMC ] [ RCm ] [ RCF ] [ RDF ] [ REK ] [ RH ] [ RLF ]
 
Rat Aortic Endothelial Cells
 (RAOEC)

Rat Aortic Endothelial Cells (RAOEC) are isolated from aorta of healthy adult rats. They are cryopreserved at third passage and can be cultured and propagated to sixth passage and beyond without losing their morphologic and phenotypic characteristics. RAOEC is a relevant in vitro model for correlating with in vivo studies in rat as an animal model. RAOEC have been used as a model for the study of Atherosclerosis1 and products synthesized by the endothelium2,3. The interaction of aortic endothelium and smooth muscle cells regulate the level and distribution of glycosaminoglycans in the arterial wall, especially in the intima4. Co-culture of aortic endothelial cells and smooth muscle cells provide an ideal in vitro model for studying the interaction between these two cells types.


Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol) Cat. No.
RAOEC Total Kit Each kit contains an ampoule of cryopreserved RAOEC (R304-05), 500 ml of Rat Endothelial Cell Growth Medium (R211-500), 100 ml of Attachment Factor Solution (123-100), and  a Subculture Reagent Kit. 1 Kit R304K-05
Cryopreserved RAOEC

(>500,000 cells in an ampoule)

Cryopreserved at third passage in Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule R304-05

Proliferating RAOEC

In Flasks

Cultured and shipped in flasks at fourth passage in Rat Endothelial Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

R305-25

R305-50

R305-75

Proliferating RAOEC  

In Multiwell Plates

Cultured and shipped in flasks at fourth passage in Rat Endothelial Cell Growth Medium.

  6 well

12 well

24 well

48 well

96 well

R305-6W

R305-12W

R305-24W

R305-48W

R305-96W

Rat Endothelial Cell Growth Medium Optimized and ready for use 500 ml R211-500
Rat Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml R211K-500
Rat Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml R210-500
Rat Endothelial Cell Growth Supplement     15 ml R211-GS
Attachment Factor Solution Promotes attachment of endothelial cells. 100 ml 123-100
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
RAOEC RNA Total RNA prepared from RAOEC

10 μg

25 μg

R304-R10

R304-R25