11. Skin Cells
 
[ HDF ] [ HEK ] [ HEM ]
Human Epidermal Melanocytes
 (HEM)

Human Epidermal Melanocytes (HEM) are primary cells isolated from normal human neonatal foreskin. HEM are cryopreserved at second passage and can be cultured and propagated at least 12 population doublings. These epidermal melanocytes produce melanin, thus a useful cell model in the study of hyperpigmentation through accentuated melanocyte proliferation and differentiation1,2, as well as progression of melanocytic neoplasia3,4.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HEM Total Kit , neonatal Each kit contains an ampoule of cryopreserved HEM (104-05), 500 ml of Melanocyte Cell Growth Medium (135-500) and a Subculture Reagent Kit (090K). 1 Kit 104K-05n
Cryopreserved HEM , neonatal

(>500,000 cells in an ampoule)

Cryopreserved at second passage in Melanocyte Basal Medium that contains 30% FBS and 10% DMSO.

1 Ampoule 104-05n

Proliferating HEM , neonatal

In Flasks

Cultured and shipped in flask at third passage in Melanocyte Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

105-25n

105-50n

105-75n

Proliferating HEM , neonatal  

In Multiwell Plates

Cultured and shipped in plates at third passage in Melanocyte Growth Medium.

  6 well 

12 well

24 well

48 well

96 well

105-6Wn

105-12Wn

105-24Wn

105-48Wn

105-96Wn

Melanocyte Growth Medium Optimized and ready for use. 500 ml 135-500
Melanocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 135K-500
Melanocyte Basal Medium Add Growth Supplement before use 500 ml 134-500
Melanocyte Growth Supplement     5 ml 135-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
Melanocyte RNA , neonatal Total RNA prepared from HEM

10 μg

25 μg

104-R10n

104-R25n