11. Skin Cells
 
[ HDF ] [ HEK ] [ HEM ]
Human Dermal Fibroblasts
 (HDF)

Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. Fibroblasts are found in all connective tissues, and they synthesize and secrete extracellular matrix proteins under cell culture conditions1. They are a well established system for in vitro analysis of fibroblast growth, migration and collagen metabolism2-5 in wound healing. Fibroblasts grown in a biodegradable mesh have been used as a living dermal replacement6,7.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HDF Total Kit

adult(a)

fetal(f)

neonatal(n)

Each kit contains an ampoule of cryopreserved HDF (106-05), 500 ml of Fibroblast Growth Medium (116-500), and a Subculture Reagent Kit (090K). 1 Kit

106K-05a

106K-05f

106K-05n

Cryopreserved HDF

adult

fetal

neonatal

(>500,000 cells in an ampoule)

Cryopreserved at the end of primary culture in Fibroblast Basal Medium that contains 10% FBS and 10% DMSO.

 1 Ampoule

106-05a

106-05f

106-05n

Proliferating HDF

In Flasks

* Specify adult (a), fetal (f), or  neonatal (n)

Cultured in flasks at first passage in Fibroblast Growth Medium and shipped in Fibroblast Transfer Medium. 

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

107-25 *

107-50 *

107-75 *

Proliferating HDF  

In Multiwell Plates

* Specify adult (a), fetal (f), or  neonatal (n)

Cultured in plates at first passage in Fibroblast Growth Medium and shipped in Fibroblast Transfer Medium. 

  6 well

12 well

24 well

48 well

96 well

107-6W *

107-12W *

107-24W *

107-48W *

107-96W *

Fibroblast Growth Medium Optimized and ready for use 500 ml 116-500
Fibroblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 116K-500
Fibroblast Basal Medium Add Growth Supplement before use 500 ml 115-500
Fibroblast Growth Supplement     15 ml 116-GS
Subculture Reagent Kit 100ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K

HDF RNA

fetal

neonatal

Total RNA prepared from HDF

10 μg  or  25 μg

10 μg  or  25 μg

106-R10f;  106-R25f

106-R10n; 106-R25n