11. Skin Cells
 
[ HDF ] [ HEK ] [ HEM ]
Human Epidermal Keratinocytes
 (HEK)

Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. HEK is a suitable in vitro model for dermal toxicology tests in screening new skin products1, and for developing dermal skin substitutes2. HEK has been used in numerous applications, including investigations of human epidermal development3, differentiation4, and cellular aspects of skin diseases5.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HEK Total Kit

adult

fetal

neonatal

Each kit contains an ampoule of cryopreserved HEK  (102-05), 500 ml of HEK Cell Growth Medium (131-500) and a Subculture Reagent Kit. 1 Kit

102K-05a

102K-05f

102K-05n

Cryopreserved HEK

adult

fetal

neonatal

(>500,000 cells in an ampoule)

Cryopreserved at the end of primary culture in Keratinocyte Basal Medium that contains 30% FBS and 10% DMSO.

1 Ampoule

102-05a

102-05f

102-05n

Proliferating HEK

In Flasks  

Cultured and shipped in flasks at first passage in Keratinocyte Serum-Free Growth Medium

*Specify adult (a), fetal (f) or neonatal (n)

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

103-25 *

103-50 *

103-75 *

Proliferating HEK

In Multiwell Plates

Cultured and shipped in plates at first passage in Keratinocyte Serum-Free Growth Medium. 

*Specify adult (a), fetal (f) or neonatal (n)

  6 well 

12 well

24 well

48 well

96 well

103-6W *

103-12W *

103-24W *

103-48W *

103-96W *

HEK Growth Medium Optimized and ready for use 500 ml 131-500
HEK Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 131K-500
HEK Basal Medium Add Growth Supplement before use 500 ml 130-500
HEK Growth Supplement      5 ml 131-GS
HEK Calcium-Free Growth Medium   500 ml 133-500
HEK Calcium-Free Growth Medium Kit   Yields 500 ml 133K-500
HEK Calcium-Free Basal Medium   500 ml 132-500
HEK Calcium-Free Growth Supplement   5 ml 133-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K

HEK RNA

adult

neonatal

Total RNA prepared from HEK

10 μg

25 μg

10 μg

25 μg

102-R10a

102-R25a

102-R10n

102-R25n