12. Umbilical Vessel Cells
 
[ HUVEC ] [ HUAEC ] [ HUASMC ] [ HUVSMC ]
Human Umbilical Vein Smooth Muscle Cells
 (HUVSMC)

Human umbilical vein smooth muscle cells (HUVSMC) are derived from the umbilical vein. They are cryopreserved at second passage. HUVSMC can be cultured and propagated 6 population doublings. 

 

Human umbilical vein actively regulates its vascular tone under the control of pO2. Vasodilation endothelial prostanoids contribute to the hypoxic vasodilation that counteracts the vasoconstricting actions of prostanoids released from HUVSMC. It was found that vasoconstricting prostanoids are released from the endothelium of the human umbilical vein at hyperoxia1. 

During preeclampsia a total loss of neuronal nitric oxide synthase (nNOS) protein expression and a significant decrease in mRNA expression were seen. It was found that such loss might alter the regulation of blood flow in the fetal and maternal placental vasculature2.

Both ouabain and marinobufagenin activate proliferation of the HUVSMC. The concentration ranges of both compounds were found to be below that necessary to induce cytoplasmic ion alterations by sodium pump inhibition3.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HUVSMC Total Kit, neonatal Each kit contains an ampoule of cryopreserved HUVSMC  (250-05n), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit. 1 Kit 250K-05n
Cryopreserved HUVSMC , neonatal (>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 250-05n

Proliferating HUVSMC , neonatal

In Flasks

 Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

251-25n

251-50n

251-75n

Proliferating HUVSMC , neonatal

In Multiwell Plates

Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.

  6 well

12 well

24 well

48 well

96 well

251-6Wn

251-12Wn

251-24Wn

251-48Wn

251-96Wn

Smooth Muscle Cell Growth Medium Optimized and ready for use . 500 ml 311-500
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500
Smooth Muscle Cell Basal Medium Add Growth Supplement before use. 500 ml 310-500
Smooth Muscle Cell Growth Supplement   30 ml 311-GS
Smooth Muscle Cell Differentiation   250 ml 311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution

100 ml

 090K