12. Umbilical Vessel Cells
 
[ HUVEC ] [ HUAEC ] [ HUASMC ] [ HUVSMC ]
Human Umbilical Artery Endothelial Cells
 (HUAEC)

Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. Arterial endothelial cells have been used as an in vitro model for the study of morphological and ultrastructural changes in the development of atherosclerosis1. The HUAEC system has also been used for the study of endothelial cell-dependent lymphocyte activation .and chemoattractant activity2,3.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HUAEC Total Kit, neonatal Each kit contains an ampoule of cryopreserved HUAEC  (202-05n), 500 ml of HUAEC Growth Medium (212-500), and a Subculture Reagent Kit. 1 Kit 202K-05n
Cryopreserved HUAEC, neonatal

(>500,000 cells in an ampoule)

Cryopreserved at first passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

 1 Ampoule 202-05n

Proliferating HUAEC, neonatal

In Flasks

Cultured in flasks at second passage in HUAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

203-25n

203-50n

203-75n

Proliferating HUAEC, neonatal

In Multiwell Plates

Cultured in plates at second passage in HUAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.

  6 well

12 well

24 well

48 well

96 well

203-6Wn

203-12Wn

203-24Wn

203-48Wn

203-96Wn
HUAEC Growth Medium Optimized and ready for use . 500 ml 212-500
HUAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 212K-500
HUAEC Basal Medium Add Growth Supplement before use 500 ml 210-500
HUAEC Growth Supplement   30 ml 212-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HUAEC RNA, neonatal Total RNA prepared from HUAEC

10 μg

25 μg

202-R10n

202-R25n