Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells are cryopreserved at the end of primary culture and can be propagated two passages prior to differentiating into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport^1,2, growth hormone enhanced lypolysis³ and obese gene expression⁴. HPAd can be fully differentiated into mature HAd in CAI’s Adipocyte Differentiation Medium. Significant differences in lipolysis and leptin production⁴ are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. We provide HPAd from subcutaneous adipose tissue at various sites and adipose depot on heart. HPAd/HAd are useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus-1⁵.