Yeast Culture
 

Amino Acid Mixtures

 

Amino acid mixtures can be made in any concentration you require. Unless otherwise indicated in the product names, our mixtures use the following recipes. For media lacking methionine, DL-homoserine (80mg/L) is substituted for L-threonine.

 

CSM mg/L

(Complete Supplement Mixture)

HSM mg/L

(Hollenberg Supplement Mixture)

BSM mg/L

(Brent Supplement Mixture)

SC mg/L

(Synthetic Complete Hopkins Mixture)

Adenine hemisulfate

10

100

40

21

L-alanine

0

0

0

85.6

L-arginine

50

100

20

85.6

L-asparagine

0

0

0

85.6

L-aspartic acid

80

50

100

85.6

L-cysteine

0

100

0

85.6

Glutamine

0

0

0

85.6

L-glutamic acid

0

0

100

85.6

Glycine

0

0

0

85.6

L-histidine

20

50

20

85.6

Myo-inositol

0

0

0

85.6

L-isoleucine

50

50

300

85.6

L-leucine

100

100

60

173.4

L-lysine

50

100

30

85.6

L-methionine

20

50

20

85.6

Para-aminobenzoic acid (PABA)

0

0

0

8.6

L-phenylalenine

50

50

50

85.6

L-proline

0

50

0

85.6

L-serine

0

50

375

85.6

L-threonine

100

100

200

85.6

L-tryptophan

50

100

40

85.6

L-tyrosine

50

50

30

85.6

Uracil

20

100

20

85.6

L-valine

140

50

150

85.6



CSM Product List

Product

Size

Cat #

g/L in

Complete Media

Complete Supplement Mixture

CSM Amino Acid Mixture

10 grams

1001-010

0.79

100 grams

1001-100

1 Amino Acid Dropout Mixtures

CSM-Ade

10 grams

1029-010

0.78

100 grams

1029-100

CSM-Arg

10 grams

1031-010

0.74

100 grams

1031-100

CSM-Asp

10 grams

1046-010

0.71

100 grams

1046-100

CSM-His

10 grams

1006-010

0.77

100 grams

1006-100

CSM-His+Ade20

10 grams

1123-010

0.78

100 grams

1123-100

CSM-His+Ade40

10 grams

1023-010

0.80

100 grams

1023-100

CSM-Leu

10 grams

1005-010

0.69

100 grams

1005-100

CSM-Leu+Ade5

10 grams

1033-010

0.69

100 grams

1033-100

CSM-Leu+Ade40

10 grams

1101-010

0.72

100 grams

1101-100

CSM-Lys

10 grams

1032-010

0.74

100 grams

1032-100

CSM-Met

10 grams

1019-010

0.75

100 grams

1019-100

CSM-Thr

10 grams

1045-010

0.69

100 grams

1045-100

CSM-Trp

10 grams

1007-010

0.74

100 grams

1007-100

CSM-Trp+Ade40

10 grams

1102-010

0.77

100 grams

1102-100

CSM-Ura

10 grams

1004-010

0.77

100 grams

1004-100

CSM-Ura+Ade40

10 grams

1103-010

0.80

100 grams

1103-100

2 Amino Acid Dropout Mixtures

CSM-Ade-Leu

10 grams

1037-010

0.68

100 grams

1037-100

CSM-Ade-Trp

10 grams

1028-010

0.73

100 grams

1028-100

CSM-Ade-Ura

10 grams

1036-010

0.76

100 grams

1036-100

CSM-Arg-His

10 grams

1034-010

0.72

100 grams

1034-100

CSM-Arg-Leu

10 grams

1042-010

0.64

100 grams

1042-100

CSM-Arg-Ura

10 grams

1022-010

0.72

100 grams

1022-100

CSM-His-Leu

10 grams

1011-010

0.67

100 grams

1011-100

CSM-His-Leu+Ade40

10 grams

1110-010

0.70

100 grams

1110-100

CSM-His-Trp

10 grams

1013-010

0.72

100 grams

1013-100

CSM-His-Trp+Ade40

10 grams

1111-010

0.75

100 grams

1111-100

CSM-His-Ura

10 grams

1009-010

0.75

100 grams

1009-100

CSM-His-Ura+Ade40

10 grams

1112-010

0.78

100 grams

1112-100

CSM-Leu-Met

10 grams

1093-010

0.65

100 grams

1093-100

CSM-Leu-Trp

10 grams

1012-010

0.64

100 grams

1012-100

CSM-Leu-Trp+Ade40

10 grams

1115-010

0.67

100 grams

1115-100

CSM-Leu-Ura

10 grams

1038-010

0.67

100 grams

1038-100

CSM-Leu-Ura+Ade40

10 grams

1113-010

0.74

100 grams

1113-100

CSM-Lys-Met

10 grams

1035-010

0.70

100 grams

1035-100

CSM-Lys-Ura

10 grams

1018-010

0.72

100 grams

1018-100

CSM-Met-Trp

10 grams

1020-010

0.70

100 grams

1020-100

CSM-Met-Ura

10 grams

1092-010

0.73

100 grams

1092-100

CSM-Trp-Ura

10 grams

1010-010

0.72

100 grams

1010-100

CSM-Trp-Ura+Ade40

10 grams

1114-010

0.75

100 grams

1114-100

3 Amino Acid Dropout Mixtures

CSM-Ade-His-Leu

10 grams

1024-010

0.66

100 grams

1024-100

CSM-Ade-Leu-Trp

10 grams

1025-010

0.63

100 grams

1025-100

CSM-Ade-Met-Ura

10 grams

1197-010

0.72

100 grams

1197-100

CSM-Ade-Trp-Ura

10 grams

1089-010

0.71

100 grams

1089-100

CSM-Arg-His-Ura

10 grams

1039-010

0.70

100 grams

1039-100

CSM-His-Leu-Trp

10 grams

1014-010

0.62

100 grams

1014-100

CSM-His-Leu-Trp+Ade40

10 grams

1030-010

0.65

100 grams

1030-100

CSM-His-Leu-Ura

10 grams

1015-010

0.65

100 grams

1015-100

CSM-His-Trp-Ura

10 grams

1016-010

0.70

100 grams

1016-100

CSM-Leu-Met-Trp

10 grams

1021-010

0.60

100 grams

1021-100

CSM-Leu-Trp-Ura

10 grams

1017-010

0.62

100 grams

1017-100

4 Amino Acid Dropout Mixtures

CSM-Ade-His-Leu-Trp

10 grams

1027-010

0.61

100 grams

1027-100

CSM-Ade-His-Leu-Ura

10 grams

1087-010

0.64

100 grams

1087-100

CSM-Ade-Met-Trp-Ura

10 grams

1196-010

0.67

100 grams

1196-100

CSM-His-Leu-Lys-Trp

10 grams

1091-010

0.57

100 grams

1091-100

CSM-His-Leu-Trp-Ura

10 grams

1002-010

0.60

100 grams

1002-100

CSM-His-Lys-Trp-Ura

10 grams

1088-010

0.65

100 grams

1088-100

5 Amino Acid Dropout Mixtures

CSM-Ade-His-Leu-Trp-Ura

10 grams

1085-010

0.59

100 grams

1085-100

CSM-His-Leu-Lys-Trp-Ura

10 grams

1086-010

0.55

100 grams

1086-100

CSM-His-Leu-Met-Trp-Ura

10 grams

1095-010

0.56

100 grams

1095-100

6 Amino Acid Dropout Mixtures

CSM-Ade-His-Leu-Lys-Trp-Ura

10 grams

1135-010

0.54

100 grams

1135-100

CSM-Ade-His-Leu-Met-Trp-Ura

10 grams

1026-010

0.55

100 grams

1026-100



SC Product List

Product

Size

Cat #

g/L in

Complete Media

Synthetic Complete Supplement Mixture

SC Amino Acid Mixture

30 grams

1300-030

2.00

1 Amino Acid Dropout Mixtures

SC-Ade

30 grams

1302-030

1.98

SC-His

30 grams

1303-030

1.92

SC-Leu

30 grams

1304-030

1.83

SC-Trp

30 grams

1305-030

1.92

SC-Ura

30 grams

1306-030

1.92

2 Amino Acid Dropout Mixtures

SC-Ade-His

30 grams

1310-030

1.89

SC-Ade-Leu

30 grams

1311-030

1.81

SC-Ade-Trp

30 grams

1312-030

1.89

SC-Ade-Ura

30 grams

1313-030

1.89

SC-His-Leu

30 grams

1314-030

1.74

SC-His-Trp

30 grams

1315-030

1.83

SC-His-Ura

30 grams

1309-030

1.83

SC-Leu-Trp

30 grams

1317-030

1.74

SC-Leu-Ura

30 grams

1318-030

1.74

SC-Trp-Ura

30 grams

1316-030

1.83

3 Amino Acid Dropout Mixtures

SC-His-Leu-Trp

30 grams

1325-030

1.66

SC-His-Leu-Ura

30 grams

1327-030

1.66

SC-His-Trp-Ura

30 grams

1326-030

1.74

SC-Leu-Trp-Ura

30 grams

1328-030

1.66

4 Amino Acid Dropout Mixtures

SC-Ade-His-Leu-Trp

30 grams

1330-030

1.64

SC-Ade-His-Leu-Ura

30 grams

1331-030

1.64

SC-Ade-His-Trp-Ura

30 grams

1332-030

1.72

SC-Ade-Leu-Trp-Ura

30 grams

1333-030

1.64

SC-Cys-Leu-Met-Ura

30 grams

1335-030

1.57

SC-His-Leu-Trp-Ura

30 grams

1334-030

1.57

5 Amino Acid Dropout Mixtures

SC-Ade-His-Leu-Trp-Ura

30 grams

1301-030

1.55

SC-Cys-His-Leu-Met-Ura

30 grams

1350-030

1.48



BSM Product List

Product

Size

Cat #

g/L in

Complete Media

BSM Supplement Mixture

BSM Amino Acid Mixture

25 grams

Inquire

1.56

1 Amino Acid Dropout Mixtures

BSM-Ura

25 grams

1382-025

1.54

SC-His

30 grams

1303-030

1.92



Complete Defined Media for S. cerevisiae

 

Complete Synthetic Defined Media contains Yeast Nitrogen Base (1.7g/L), ammonium sulfate (5g/L), dextrose (20g/L) and a Complete Supplement Mixture (CSM) of amino acids minus those indicated in the product name. Broth (SD) and Agar (SDA) mixtures are available.

 

Product

Size

Cat #

g/L

Complete Defined Media

SD Medium

10 x 0.5 L pouches

1701-300

27.49

500 grams

1701-500

SDA Medium Agar

10 x 0.5 L pouches

1702-300

44.49

500 grams

1702-500

1 Amino Acid Dropout Media

SDA-Ade

10 x 0.5 L pouches

1712-300

44.48

10 grams

1712-010

500 grams

1712-500

SD-Ade

10 x 0.5 L pouches

1711-300

27.48

500 grams

1711-500

SDA-His

10 x 0.5 L pouches

1706-300

44.47

10 grams

1706-010

500 grams

1706-500

SD-His

10 x 0.5 L pouches

1705-300

27.47

500 grams

1705-500

SDA-Leu

10 x 0.5 L pouches

1708-300

44.39

10 grams

1708-010

500 grams

1708-500

SD-Leu

10 x 0.5 L pouches

1707-300

27.39

10 grams

1707-010

500 grams

1707-500

SDA-Trp

10 x 0.5 L pouches

1710-300

44.44

10 grams

1710-010

500 grams

1710-500

SD-Trp

10 x 0.5 L pouches

1709-300

27.44

10 grams

1709-010

500 grams

1709-500

SDA-Ura

10 x 0.5 L pouches

1704-300

44.47

10 grams

1704-010

500 grams

1704-500

SD-Ura

10 x 0.5 L pouches

1703-300

27.47

10 x 1.0 L pouches

1703-400

500 grams

1703-500

2 Amino Acid Dropout Media

SDA-His-Leu

10 x 0.5 L pouches

1714-300

43.37

500 grams

1714-500

SD-His-Leu

10 x 0.5 L pouches

1713-300

27.37

500 grams

1713-500

SDA-His-Trp

10 x 0.5 L pouches

1716-300

44.42

500 grams

1716-500

SD-His-Trp

10 x 0.5 L pouches

1715-300

27.42

500 grams

1715-500

SDA-His-Ura

10 x 0.5 L pouches

1718-300

44.45

500 grams

1718-500

SD-His-Ura

10 x 0.5 L pouches

1717-300

27.45

500 grams

1717-500

SDA-Leu-Trp

10 x 0.5 L pouches

1720-300

44.34

10 grams

1720-010

500 grams

1720-500

SD-Leu-Trp

10 x 0.5 L pouches

1719-300

27.34

10 grams

1719-010

500 grams

1719-500

SDA-Leu-Ura

10 x 0.5 L pouches

1722-300

44.37

500 grams

1722-500

SD-Leu-Ura

10 x 0.5 L pouches

1721-300

27.37

500 grams

1721-500

SDA-Trp-Ura

10 x 0.5 L pouches

1724-300

44.42

500 grams

1724-500

SD-Trp-Ura

10 x 0.5 L pouches

1723-300

27.42

500 grams

1723-500

3 Amino Acid Dropout Media

SDA-His-Leu-Trp

10 x 0.5 L pouches

1726-300

44.32

500 grams

1726-500

SD-His-Leu-Trp

10 x 0.5 L pouches

1725-300

27.32

500 grams

1725-500

SDA-His-Leu-Ura

10 x 0.5 L pouches

1728-300

44.35

500 grams

1728-500

SD-His-Leu-Ura

10 x 0.5 L pouches

1727-300

27.35

500 grams

1727-500

SDA-His-Trp-Ura

10 x 0.5 L pouches

1734-300

44.40

500 grams

1734-500

SD-His-Trp-Ura

10 x 0.5 L pouches

1733-300

27.40

500 grams

1733-500

SDA-Leu-Trp-Ura

10 x 0.5 L pouches

1730-300

44.32

10 x 1.0 L pouches

1730-400

500 grams

1730-500

SD-Leu-Trp-Ura

10 x 0.5 L pouches

1729-300

27.32

500 grams

1729-500

4 Amino Acid Dropout Media

SDA-Ade-His-Leu-Trp

10 x 0.5 L pouches

1736-300

44.31

500 grams

1736-500

SD-Ade-His-Leu-Trp

10 x 0.5 L pouches

1735-300

27.31

500 grams

1735-500

SDA-His-Leu-Trp-Ura

10 x 0.5 L pouches

1732-300

44.30

500 grams

1732-500

SD-His-Leu-Trp-Ura

10 x 0.5 L pouches

1731-300

27.30

500 grams

1731-500

 

Dropout Base for S. cerevisiae

 

Dropout Base contains Yeast Nitrogen Base (1.7g/L), ammonium sulfate (5g/L) and dextrose (20g/L). If another sugar is indicated in the product name, that sugar is present at 20g/L and dextrose/glucose is not present. Broth (DOB) and Agar (DOBA) mixtures are available.

 

Product

Size

Cat #

g/L in Complete Media

Dropout Base

250 grams

1650-250

26.70

500 grams

1650-500

1 kg

1650-1kg

Dropout Base with Agar

250 grams

1651-250

43.70

500 grams

1651-500

1 kg

1651-1kg

Dropout Base w/ 2% Galactose

250 grams

1652-250

26.70

500 grams

1652-500

1 kg

1652-1kg

Dropout Base w/ Agar, 2% Galactose

250 grams

1653-250

43.70

500 grams

1653-500

1 kg

1653-1kg

Dropout Base w/ 2% Raffinose

250 grams

1654-250

26.70

500 grams

1654-500

1 kg

1654-1kg

Dropout Base w/ Agar, 2% Raffinose

250 grams

1655-250

43.70

500 grams

1655-500

1 kg

1655-1kg

Dropout Base w/ 2% Galactose, 1% Raffinose

250 grams

1656-250

36.70

500 grams

1656-500

1 kg

1656-1kg




Yeast Nitrogen Base for S. cerevisiae

Yeast Nitrogen Base (YNB) contains salts, vitamins and trace elements as defined below. Add 1.7 g/L of YNB to ammonium sulfate (5g/L), dextrose (20g/L) and an amino acid mix to make a complete media.

 

Potassium phosphate (1000 mg/L)

Inositol (2 mg/L)

Copper sulfate (0.04 mg/L)

Magnesium sulfate (500 mg/L)

Niacin (0.4 mg/L)

Potassium iodide (0.1 mg/L)

Sodium chloride (100 mg/L)

PAA (0.2 mg/L)

Ferric chloride (0.2 mg/L)

Calcium chloride (100 mg/L)

Pyridoxine (0.4 mg/L)

Manganese sulfate (0.4 mg/L)

Biotin (0.002 mg/L)

Riboflavin (0.2 mg/L)

Sodium molybdate (0.2 mg/L)

Pantothenate, calcium (0.4 mg/L)

Thiamine (0.4 mg/L)

Zinc sulfate (0.4 mg/L)

Folic acid (0.002 mg/L)

Boric acid (0.5 mg/L)

 

 

Product

Size

Cat #

g/L in Complete Media

Yeast Nitrogen Base (YNB)

250 grams

1500-250

1.70

500 grams

1500-500

YNB-Cu-Fe

250 grams

1526-250

1.70

500 grams

1526-500

YNB-Calcium

250 grams

1529-250

1.61

500 grams

1529-500

Yeast Nitrogen Base with Ammonium Sulfate (YNB+Nitrogen)

250 grams

1501-250

6.70

500 grams

1501-500

YNB+Nitrogen-Cu-Fe

250 grams

1527-250

6.70

500 grams

1527-500

YNB+Nitrogen-Calcium

250 grams

1528-250

6.61

500 grams

1528-500

YPD (YEPD) Medium

YPD (YEPD) Medium contains peptone (20g/L), yeast extract (10g/L) and dextrose (20g/L).

 

Product

Size

Cat #

g/L

YPD Medium

250 grams

1875-250

50.00

500 grams

1875-500

1 kg

1875-1kg

10 x 1.0 L

1875-400

YPD Agar

250 grams

1876-250

67.00

500 grams

1876-500

1 kg

1876-1kg

10 x 1.0 L

1876-400

YEP Medium

YEP Medium contains peptone (20g/L) and yeast extract (10g/L).

 

Product

Size

Cat #

g/L

YEP Medium

250 grams

1877-250

30.00

500 grams

1877-500

1 kg

1877-1kg

10 x 1.0 L

1877-400

Sporulation Media for S. cerevisiae

Sporulation Media contains potassium acetate (20g/L), yeast extract (1g/L) and agar (17g/L).

 

Product

Size

Cat #

g/L

Sporulation Medium Agar

250 grams

1822-250

38.00

500 grams

1822-500

Complete Defined Media for S. pombe

EMM Media contains salts, vitamins, trace elements and other components as defined below. Use 32.2 g/L.

 

 

Potassium phthalate (3 g/L)

Na2SO4 (0.04 g/L)

ZnSO4 (0.4 mg/L)

Na2HPO4 (2.2 g/L)

Pantothenic acid (1 mg/L)

FeCl2 (0.2 mg/L)

NH4Cl (5 g/L)

Nicotinic acid (10 mg/L)

Molybdic acid (40 μg/L)

Dextrose (20 g/L)

Myo-inositol (10 mg/L)

Potassium Iodide (0.1 mg/L)

MgCl2 (1.05 g/L)

Biotin (1 mg/L)

CuSO4 (40 μg/L)

CaCl2 (14.7 mg/L)

Boric acid (0.5 mg/L)

Citric acid (1 mg/L)

KCl (1 g/L)

MnSO4 (0.4 mg/L)

 

 

Product

Size

Cat #

g/L

EMM Medium

10 x 0.5 L pouches

2005-300

32.20

10 x 1.0 L pouches

2005-400

250 grams

2005-250

500 grams

2005-500

1 kg

2005-1kg

2 kg

2005-2kg

MMA Agar contains salts, vitamins, trace elements and other components as defined below. Use 33.72 g/L.

 

 

Agar Y, (17 g/L)

(NH4)2SO4 (5.0 g/L)

ZnSO4 (0.4 mg/L)

Potassium phosphate (1 g/L)

Pantothenic acid (1 mg/L)

FeCl3 (0.2 mg/L)

Dextrose (10 g/L)

Nicotinic acid (10 mg/L)

Molybdic acid (200 μg/L)

 

Myo-inositol (10 mg/L)

Potassium Iodide (0.1 mg/L)

MgSO4 (0.5 g/L)

Biotin (10 ug/L)

CuSO4 (40 μg/L)

CaCl2 (0.1 g/L)

Boric acid (0.5 mg/L)

 

NaCl (0.1 g/L)

MnSO4 (0.4 mg/L)

 

 

Product

Size

Cat #

g/L

MMA Agar

10 x 0.5 L pouches

2015-300

33.72

10 x 1.0 L pouches

2015-400

250 grams

2015-250

500 grams

2015-500

1 kg

2015-1kg

Rich Undefined Growth Media for S. pombe

YES Media contains 5 g/L yeast extract, 30 g/L glucose and 225 mg/L each of adenine, histidine, leucine, uracil and lysine. Use 36.13 g/L.YES Agar contains the above plus 17 g/L Agar-Y. Use 53.13 g/L.

 

Product

Size

Cat #

g/L

YES Medium

10 x 0.5 L pouches

2009-300

36.13

10 x 1.0 L pouches

2009-400

250 grams

2009-250

500 grams

2009-500

1 kg

2009-1kg

YES Medium Agar

10 x 0.5 L pouches

2010-300

53.13

10 x 1.0 L pouches

2010-400

250 grams

2010-250

500 grams

2010-500

1 kg

2010-1kg

Amino Acid Supplements for S. pombe

SP Supplements contain 50 mg/L each of adenine, histidine, leucine, uracil and lysine.

 

Product

Size

Cat #

g/L

SP Complete Supplements

 

10 Grams

2200-010

0.25

100 Grams

2200-100

SP Supplements -Lys

 

10 Grams

2202-010

0.20

100 Grams

2202-100

SP Supplements -Leu-Ura

 

10 Grams

2201-010

0.15

100 Grams

2201-100

Yeast Culture Grade Supplements and Additives

 

Product

Size

Cat #

Agar

Agar

500 grams

1910-500

1 kg

1910-1kg

Amino Acids

4-aminobenzoic acid (PABA)

10 grams

1996-010

DL-homoserine

5 grams

1997-005

L-alanine

10 grams

1970-010

L-arginine

10 grams

1971-010

L-asparagine

10 grams

1972-010

L-aspartic acid

10 grams

1973-010

L-cysteine

10 grams

1974-010

L-glutamic acid

10 grams

1976-010

L-glutamine

10 grams

1975-010

L-glycine

10 grams

1977-010

L-histidine

10 grams

1978-010

L-isoleucine

10 grams

1979-010

L-leucine

10 grams

1980-010

L-lysine

10 grams

1981-010

L-methionine

10 grams

1982-010

L-phenylalanine

10 grams

1983-010

L-proline

10 grams

1984-010

L-serine

10 grams

1985-010

L-threonine

10 grams

1986-010

L-tryptophan

10 grams

1987-010

L-tyrosine

10 grams

1988-010

L-valine

10 grams

1989-010

Sugars

Galactose, 20% (sterile)

500 ml

1901-500

Glucose, 20% (sterile)

500 ml

1900-500

Raffinose, 20% (sterile)

500 ml

1902-500

Galactose, powder

1 kg

1908-1KG

Galactose, powder

500 g

1908-500

Galactose, powder

250 g

1908-250

Glucose, powder

500 g

1907-500

Glucose, powder

250 g

1907-250

Raffinose, powder

500 g

1909-500

Raffinose, powder

250 g

1909-250

Raffinose, powder

25g

1909-025

Supplements

3-AT (3 amino-1,2,4-triazole)

10 grams

1920-010

3-AT (3 amino-1,2,4-triazole)

50 grams

1920-050

5-FOA (5-Fluoroorotic acid)

1 gram

1921-001

Adenine hemisulfate

10 grams

1905-010

Inositol

10 grams

1995-010

Uracil

10 grams

1906-010