10. Placental Cells
 
Human Placental Epithelial Cells
 
(HPlEpC)

Human Placental Epithelial Cells ( HPlEpC ) are isolated from normal human amnion. They are cryopreserved at the end of primary culture. These epithelial cells proliferate well when they are thawed and plated from cryopreservation, but they do not plate and proliferate after trypsinization.

HPlEpC has been used to study the secretion of activin A1, the neural formation during early fetal development2, and the antimicrobial and endotoxin-neutralizing barrier effect of Histones H2A and H2B against microorganism3. Prostaglandin E2 induces high level of amniotic epithelial cell death via necrosis rather than apoptosis4. Matrix metalloproteinases?9 (MMP-9) localized mainly in amnion epithelia. An increase in MMP-9 expression may contribute to degradation of extracellular matrix in fetal membrane and placenta, thereby facilitate fetal membrane rupture and placental detachment5.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HPlEpC Total Kit

fetal

neonatal

Each kit contains an ampoule of cryopreserved HPlEpC (230-05), 500 ml of Placental Epithelial Cell Growth Medium (215-500), and a Subculture Reagent Kit (090K). 1 Kit

230K-05f

230K-05n

Cryopreserved HPlEpC

fetal

neonatal

(>500,000 cells in an ampoule)

Cryopreserved at primary passage in Placental Epithelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

230-05f

230-05n

Proliferating HPlEpC

fetal

In Flasks

Cultured in flasks at first passage in Placental Epithelial Cell Growth Medium

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

231-25f

231-50f

231-75f

Proliferating HPlEpC

neonatal

In Flasks

Cultured in flasks at first passage in Placental Epithelial Cell Growth Medium

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

231-25n

231-50n

231-75n

Proliferating HPlEpC

fetal

In Multiwell Plates

Cultured in plates at first passage in Placental Epithelial Cell Growth Medium

  6 well 

12 well

24 well

48 well

96 well

231-6Wf

231-12Wf

231-24Wf

231-48Wf

231-96Wf

Proliferating HPlEpC

neonatal

In Multiwell Plates

Cultured in plates at first passage in Placental Epithelial Cell Growth Medium

  6 well 

12 well

24 well

48 well

96 well

231-6Wn

231-12Wn

231-24Wn

231-48Wn

231-96Wn

Placental Epithelial Cell Growth Medium Optimized and ready for use 500 ml 215-500
Placental Epithelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 215K-500
Placental Epithelial Cell Basal Medium Add Growth Supplement before use 500 ml 214-500
Placental Epithelial Cell Growth Supplement      5 ml 215-GS
Subculture Reagent Kit 100ml each of HBSS, Trypsin/EDTA & Neutralizing Solution 100ml 090K

HPlEpC RNA

fetal

neonatal

Total RNA prepared from HPlEpC 10 μg

230-R10f

230-R10n

HPlEpC RNA

fetal

neonatal

Total RNA prepared from HPlEpC 25 μg

230-R25f

230-R25n