9. Mammary Cells
 
Human Mammary Epithelial Cells
 (HMEpC)

Human Mammary Epithelial Cells (HMEpC) are derived from normal adult mammary glands. They are cryopreserved at fifth passage. HMEpC can be cultured and propagated 16 population doublings. It was shown that normal HMEpC have a high amount of cyclin-dependent kinase 6 activity, which restrains HMEpC proliferation, and loss or down-regulation of cdk 6 may play a role in breast tumor development1. Transforming HMEpC to immortality and malignancy was a useful model for studying early breast carcinogenesis2. Autocrine colony-stimulating factor receptors may contribute to invasive progression in epithelial cancer was demonstrated in a 3-dimensional culture of HMEpC3.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.
HMEpC Total Kit, adult Each kit contains an ampoule of cryopreserved HMEpC (830-05a), 500 ml of Mammary Epithelial Cell Growth Medium (815-500), and a Subculture Reagent Kit (090K). 1 Kit 830K-05a
Cryopreserved HMEpC , adult

(>500,000 cells in an ampoule)

Cryopreserved in ampoules at fifth passage in Mammary Epithelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 830-05a

Proliferating HMEpC , adult

In Flasks

Cultured and shipped in flasks at sixth passage in Mammary Epithelial Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

831-25a

831-50a

831-75a

Proliferating HMEpC , adult  

In Multiwell Plates

 

Proliferating HMEpC seeded in inserts are available upon request.

 Cultured and shipped in flasks at sixth passage in Mammary Epithelial Cell Growth Medium.

 6 well 

12 well

24 well

48 well

96 well

831-6Wa

831-12Wa

831-24Wa

831-48Wa

831-96Wa
Mammary Epithelial Cell Growth Medium Optimized and ready for use 500 ml 815-500
Mammary Epithelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 815K-500
Mammary Epithelial Cell Basal Medium Add Growth Supplement before use 500 ml 814-500
Mammary Epithelial Cell Growth Supplement   5 ml 815-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
HMEpC RNA , adult Total RNA prepared from HMEpC

10 μg

25 μg

830-R10a

830-R25a