8. Lung Cells
 
Human Lung Fibroblasts
 
(HLF)

Human Lung Fibroblasts (HLF) are derived from normal human lung parenchyma. They are cryopreserved at first passage and can be cultured and propagated at least 12 population doublings.

Studies show HLF play a pivotal role in airway inflammation and it may be an important target for drugs used in asthma1. Fibroblast-based assay was used for screening of new antimicrobial drugs2. Human cytomegalovirus (HCMV) was found to completely down-regulate EGF receptor on the surface of fetal HLF3. Thrombin contributes to bronchoalveola lavage fluid mitogenicity in lung disease of the premature infant4. Pseudomonas autoinducer N-(3-oxododecanoyl) homoserine lactone induces cyclooxygenase-2 and prostaglandin E2 production in HLF important for inflammation and immune defenses5. Studies also show cigarette smoke extract added to the medium induces growth inhibition of HLF6.


Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol) Cat. No.

HLF Total Kit

adult fetal

Each kit contains an ampoule of cryopreserved HLF  (506-05a or 506-05f), 500 ml of HLF Growth Medium (516-500), and a Subculture Reagent Kit (090K). 1 Kit

506K-05a

506K-05f

Cryopreserved HLF

adult fetal

(>500,000 cells in an ampoule)

Cryopreserved at first passage in HLF Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

506-05a

506-05f

Proliferating HLF, adult

In Flasks

Cultured and shipped in flasks at second passage in HLF Growth Medium .

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

507-25a

507-50a

507-75a

Proliferating HLF, fetal

In Flasks

Cultured and shipped in flasks at second passage in HLF Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

507-25f

507-50f

507-75f

Proliferating HLF, adult

In Multiwell Plates

Cultured and shipped in plates at second passage in HLF Growth Medium

  6 well 

12 well

24 well

48 well

96 well

507-6Wa

507-12Wa

507-24Wa

507-48Wa

507-96Wa

Proliferating HLF, fetal

In Multiwell Plates

Cultured and shipped in plates at second passage in HLF Growth Medium

  6 well 

12 well

24 well

48 well

96 well

507-6Wf

507-12Wf

507-24Wf

507-48Wf

507-96Wf

HLF Growth Medium Optimized and ready for use 500 ml 516-500
HLF Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 516K-500
HLF Basal Medium Add Growth Supplement before use 500 ml 515-500
HLF Growth Supplement     50 ml 516-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K

HLF RNA

adult fetal

Total RNA prepared from HLF 10 μg

506-R10a

506-R10f