3. Bronchus/Trachea Cells
 
Pre-screened Human Skeletal Muscle Cells
 
(HBEpC/HTEpC)
Human Bronchial/Tracheal Epithelial Cells (HBEpC/HTEpC ) are derived from the surface epithelium of normal human bronchi/trachea. They are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings. Airway epithelial cells are targets in asthma, viral infection, and fibrotic lung disease. These cells have been used to study the regulation of intracellular pH1, the stimulant effect of IL-1 on airway epithelial cell growth2, and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells3.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi
Product Name Product Description Size (vol) Cat. No.

HBEpC Total Kit , adult

HTEpC Total Kit, adult

Each kit contains an ampoule of cryopreserved HBEpC/HTEpC (502-05a or 504-05a), 500 ml of Bronchial/Tracheal Epithelial Cell Growth Medium (511-500), and a Subculture Reagent Kit (090K). 1 Kit 502K-05a

504K-05a
Cryopreserved HBEpC, adult

Cryopreserved HTEpC, adult

(>500,000 cells in an ampoule)

Cryopreserved at first passage in Bronchial/Tracheal Epithelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule 502-05a

504-05a

Proliferating HBEpC, adult

In Flasks

Cultured and shipped in flasks at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

503-25a

503-50a

503-75a

Proliferating HTEpC, adult

In Flasks

Cultured and shipped in flasks at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium.

T-25 Flask

2 T-25 Flasks

T-75 Flask or 3 T-25 Flasks

505-25a

505-50a

505-75a

Proliferating HBEpC, adult

In Multiwell Plates

Cultured and shipped in plates at second  passage in Bronchial/Tracheal Epithelial Cell Growth Medium.

6 well

12 well

24 well

48 well

96 well

503-6Wa

503-12Wa

503-24Wa

503-48Wa

503-96Wa

Proliferating HTEpC, adult

In Multiwell Plates

Cultured and shipped in plates at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium.

6 well 

12 well

24 well

48 well

96 well

505-6Wa

505-12Wa

505-24Wa

505-48Wa

505-96Wa

HBEpC/HTEpC Growth Medium Optimized and ready for use. 500 ml 511-500
HBEpC/HTEpC Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 511K-500
HBEpC/HTEpC Basal Medium Add Growth Supplement before use 500 ml 510-500
HBEpC/HTEpC Growth Supplement   5 ml 511-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution. 100 ml 090K
HBEpC RNA, adult

HTEpC RNA, adult

Total RNA prepared from HBEpC

Total RNA prepared from HTEpC

10 μg

10 μg
502-R10a

504-R10a
HBEpC RNA, adult

HTEpC RNA, adult

Total RNA prepared from HBEpC

Total RNA prepared from HTEpC

25 μg

25 μg
502-R25a

504-R25a