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3. Bronchus/Trachea Cells

Pre-screened Human Skeletal Muscle Cells
(HBEpC/HTEpC)

Human Bronchial/Tracheal Epithelial Cells (HBEpC/HTEpC ) are derived from the surface epithelium of normal human bronchi/trachea. They are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings. Airway epithelial cells are targets in asthma, viral infection, and fibrotic lung disease. These cells have been used to study the regulation of intracellular pH¹, the stimulant effect of IL-1 on airway epithelial cell growth², and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells³.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi

Product Name	Product Description	Size (vol)	Cat. No.
HBEpC Total Kit , adult HTEpC Total Kit, adult	Each kit contains an ampoule of cryopreserved HBEpC/HTEpC (502-05a or 504-05a), 500 ml of Bronchial/Tracheal Epithelial Cell Growth Medium (511-500), and a Subculture Reagent Kit (090K).	1 Kit	502K-05a 504K-05a
Cryopreserved HBEpC, adult Cryopreserved HTEpC, adult	(>500,000 cells in an ampoule) Cryopreserved at first passage in Bronchial/Tracheal Epithelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	502-05a 504-05a
Proliferating HBEpC, adult In Flasks	Cultured and shipped in flasks at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	503-25a 503-50a 503-75a
Proliferating HTEpC, adult In Flasks	Cultured and shipped in flasks at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	505-25a 505-50a 505-75a
Proliferating HBEpC, adult In Multiwell Plates	Cultured and shipped in plates at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	503-6Wa 503-12Wa 503-24Wa 503-48Wa 503-96Wa
Proliferating HTEpC, adult In Multiwell Plates	Cultured and shipped in plates at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	505-6Wa 505-12Wa 505-24Wa 505-48Wa 505-96Wa
HBEpC/HTEpC Growth Medium	Optimized and ready for use.	500 ml	511-500
HBEpC/HTEpC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500 ml	511K-500
HBEpC/HTEpC Basal Medium	Add Growth Supplement before use	500 ml	510-500
HBEpC/HTEpC Growth Supplement		5 ml	511-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution.	100 ml	090K
HBEpC RNA, adult HTEpC RNA, adult	Total RNA prepared from HBEpC Total RNA prepared from HTEpC	10 μg 10 μg	502-R10a 504-R10a
HBEpC RNA, adult HTEpC RNA, adult	Total RNA prepared from HBEpC Total RNA prepared from HTEpC	25 μg 25 μg	502-R25a 504-R25a